In order to use the QuantiIF macro, it is necessary to save the QuantIF.ijm file in the "Plugins" folder of ImageJ. (Open leaf image via Select File → Open Samples → Leaf) Convert scanned color image of leaf to grayscale: Image → Type → 8-bit Set measurement scale: Draw a line over a 50 mm section of the ruler then Analyze → Set Scale In . NIH ImageJ and Slice-O-Matic Computed Tomography Imaging Software to ... Use of ImageJ software for histomorphometric evaluation of normal and ... Medina (2013) 1. threshold - Measuring Intensity Using ImageJ - Stack Overflow The uses of ImageJ ImageJ is a great tool to process images and perform analysis. The depicted procedures were exemplified by analysing . PDF How to Measure Leaf Disease Damage Using Image Analysis in ImageJ The use of image analysis software to quantify porosity. Using ImageJ to quantify protein bands on a PAGE gel. - YouTube 2. Click the Measure function to obtain lengths and widths. Biologists who are unsure of which distribution to choose should opt for Fiji. Therefore we recommend conversion to .bmp format. For instance, in the sample image, if no selection is made, ImageJ will caculate all the red area. Subsequently, a 16-bit histo-gram of the Hounsfield unit density scores was generated and used to calculate the total cross-sectional area for each ROI. This process may be used to highlight specific data from the stack and is accessed using "Image/Stacks/Z Project…". DiameterJ is able to analyze an image and find the diameter of nanofibers or microfibers at every pixel along a fibers axis and produces a histogram of these diameters. Record mean intensity data. 3. Subsequently, a 16-bit histo-gram of the Hounsfield unit density scores was generated and used to calculate the total cross-sectional area for each ROI. This will open a new image. 2016; Mutka and Bart 2015; Sibiya and Sumbwanyambe 2019; Xie et al. Step 2. Tick "Dark background" and select "Default" and Red" from the pulldown menus.